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The synthesis of haptens of 15α-hydroxyestrone, 15α-hydroxyestradiol, and 15α-hydroxyestriol (estetrol) was undertaken, to obtain specific antisera required for enzyme immunoassay. 3-(1-Carboxypropyl) ethers of these 15α-hydroxyestrogens were prepared and conjugated with bovine serum albumin and horseradish peroxidase. The specificity of antisera elicited against bovine serum albumin conjugates was...
3-(1-Carboxypropyl) ether derivatives of 15α-hydroxyestradiol 15-N-acetylglucosaminide (15α-OHE 2 15NAG) and 15α-hydroxyestriol (E 4 ) 15NAG were synthesized and conjugated with bovine serum albumin. Antisera elicited in rabbits possessed high affinity and specificity for the 15α-hydroxyestrogen (15α-OHEs) 15NAG, exhibiting no significant cross-reactivity with 15α-OHEs and their positional...
17α-estradiol 17-N-acetylglucosaminide (17αE 2 17NAG) is an estrogen metabolite hitherto obtained only in rabbits. To gain insight into this unique conjugate, an enzyme immunoassay (EIA) was established by using antiserum elicited against 3-[3-(1-carboxypropyl)] ether of 17αE 2 17NAG-bovine serum albumin conjugate; horseradish peroxidase, as a label; and 3,3',5,5'-tetramethylbenzidine,...
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