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We reported that the phototrophic metabolism via plasmid‐originated Gloeobacter rhodopsin(GR)‐expression is improved in Escherichia coli ET5 harboring pKJ606‐GR by a genomic point mutation (dgcQC1082A) encoding a transmembrane cell signaling protein (Microb. Cell Fact. 16:111, 2017). Another evolved descendant is isolated from the chemostat, and the genome variation of the strain named ET8 harboring...
Background The expression of the Gloeobacter rhodopsin (GR) in a chemotrophic Escherichia coli enables the light-driven phototrophic energy generation. Adaptive laboratory evolution has been used for acquiring desired phenotype of microbial cells and for the elucidation of basic mechanism of molecular evolution. To develop an optimized strain for the artificially acquired phototrophic metabolism,...
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