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Further investigation on the roots of Aconitum piepunense led to the isolation of a novel bis‐diterpenoid alkaloid designated as piepunine (1). Its structure was established by extensive interpretation of its 1D‐ and 2D‐NMR data, high‐resolution ESI‐MS, and IR spectra. Piepunine represents the first example of an atisine–denudatine‐type bis‐diterpenoid alkaloids.
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