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The possible local peripheral and spinal (intrathecal) antinociceptive effect of Na + –K + –2Cl − cotransporter (NKCC) inhibitors was investigated in the rat formalin test. Nociceptive flinching behavior induced by formalin (1%) injection in the hind paw was assessed following administration of cotransporter inhibitors. Local peripheral pretreatment in the ipsilateral paw with...
Experimental maneuvers known to increase cellular cAMP levels evoked a stimulation in the K + influx across the anterior surfaces of isolated rabbit lenses, as measured by 86 Rb + uptake. For this, the lenses were mounted in a modified Ussing-type chamber and exposed to the radiolabel under short-circuit conditions. The enhanced, cAMP-elicited flux was attributed to the...
Earlier work from this laboratory demonstrated a bumetanide-inhibitable K+ uptake activity in cultured bovine lens epithelial cells, but not at the anterior surfaces of intact bovine lenses isolated in an Ussing-type chamber. Presently the distribution of the bumetanide-sensitive Na+-K+-2Cl− cotransporter within the lens was re-examined. To complement previous results, 86Rb+ uptake experiments were...
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