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When a larva of the red flour beetle Tribolium freemani (Hinton) was introduced into a vial, the larva pupated within 6 days, whereas when two larvae were introduced, the larvae pupated in 12 days. Crowded larvae delayed production of juvenile-hormone esterase (JHE) and release of ecdysteroids, as measured by radioimmunoassay, compared with the isolated larvae. The highest JHE activity was observed...
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