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Heterologous expression is an efficient strategy for target protein production. Dlt operon plays the important role in the d-alanylation of lipoteichoic acid, which might affect the net negative charge of cell wall for protein secretion. In this study, dlt operon was deleted to improve the target protein production, and nattokinase, α-amylase and β-mannanase with different isoelectric points (PIs)...
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