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In this study, we evaluated various parameters of sperm cryopreservation in two livebearers, guppies (Poecilia reticulata) and black mollies (P. latipinna). Our results suggested a common freezing protocol for the guppies and mollies: suspend sperm in Hanks’ balanced salt solution (HBSS) at 300mOsm/kg, use 14% glycerol as cryoprotectant, cool at 25°C/min, and thaw at 40°C in a water bath for 7s. Live...
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