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An improved, rapid and inexpensive gel mobility shift assay was developed for the screening of anthracycline antibiotics. The assay based on the intercalation activity of these molecules into dsDNA was used to assess the activity of partially purified antibiotics. Detection limits were of 0.1 ng ml−1 with an average run time of 2 h. The assay is potentially useful for high throughput screening in...
A lactose-free, low-cost culture medium for the production of β-d-galactosidase by Kluyveromyces marxianus was formulated. At high aeration rates (2.2 vvm) and concentrations of 100 g sugar cane molasses l−1 as carbon source and 100 g corn steep liquor l−1 as vitamin and nitrogen source an enzyme production of 708 U l−1 h was achieved. This was 20% higher than using a medium that contained lactose...
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