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Most natural conjugative IncF plasmids encode a fertility inhibition system that represses transfer gene expression in the majority of plasmid-carrying cells. The successful spread of these plasmids in clinically relevant bacteria has been suggested to be supported by a transitory derepression of transfer gene expression in newly formed transconjugants. In this study, we aimed to monitor the extent...
The intergenic region linking conjugative transfer and replication copy control modules of IncF plasmids shows conservation of gene homology and organization. Genes distal to finO are coordinately expressed with the upstream transfer operon encoding the majority of conjugation genes in related plasmids. Here we investigate potential functions for these genes in copy number control and in processes...
finO is the final gene in the 35.4kb transfer operon of IncFI plasmid F that is known to be involved in self-conjugative transfer. The genetic region distal to finO separates the conjugation and replication control modules of IncFII plasmid R100 and carries uncharacterized genes not found in plasmid F. However, comparison of the R100 gene organization with database entries of F-like plasmids suggests...
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