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The sensitivity of a coulometric sensing device was enhanced by combining it with metallization and used for the detection of proteins. Alpha-1-fetoprotein (AFP) was used as a model analyte. Microfabricated flow channels were used to process solutions. Hydrogen peroxide produced by the enzymatic reaction of glucose oxidase (GOD) bound to protein-antibody complexes was oxidized on a platinum electrode...
Electrochemical microdevice for the determination of proteins was realized based on coulometric detection of an enzymatic reaction product (H2O2) in a solution plug coupled with silver metallization. Significant enhancement of sensitivity and lowering the detection limit was achieved by separating the oxidation and reduction reactions in two flow channels. The amount of the deposited silver was measured...
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