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Determination of the activity of an enzyme in a solution of nL order was conducted using an electrochemical device with a plug-based microfluidic processing function. β-galactosidase (β-Gal) and adenosine triphosphatase (ATPase) were used as analyte enzymes. β-Gal is often used to be conjugated with antibodies, whereas the latter plays critical roles in cell functions. The activity of the enzymes...
A plug-based electrochemical microdevice was fabricated for on-site determination of freshness or ageing of foods. As indicators to represent the state, the K-value or the concentration of inosine monophosphate (IMP) was used. Two sets of thin-film three-electrode systems were located in a flow channel. Mechanisms to form liquid plugs, to merge the plugs, and to place the plug on the electrode area...
Nitrification activity of a microbial complex was monitored continuously using a microdevice with integrated NH4+ ion-selective electrodes. The device consisted of a glass substrate with sensing electrodes, polydimethylsiloxane (PDMS) substrates with chambers for cell culture and an internal electrolyte solution, and a polymethylmethacrylate (PMMA) substrate with NH4+ ion-selective membranes. To stabilize...
A microfluidic device for adenosine triphosphate (ATP) was checking of fish freshness. Sample solutions containing ATP and the other solution containing an enzyme substrate were handled in the form of plugs formed in a network of flow channels. To simplify the structure and procedure for mixing solutions, a wider portion was formed in a flow channel to merge the plugs. A row of plugs were ejected...
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