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6-Rhodamine B amine functions as a highly sensitive fluorescence derivatization reagent for mono- and oligosaccharides; it reacts with the reducing end of saccharides under acidic conditions. The fluorescent derivatives of five monosaccharides can be separated within 25min by reversed-phase liquid chromatography with isocratic elution. The detection limits (S/N=3) for mono-, di-, and oligosaccharides...
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