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Experimental determination of fluxes by 13C‐tracers relies on detection of 13C‐patterns in metabolites or by‐products. In the field of 13C metabolic flux analysis, the most recent developments point toward recording labeling patterns by liquid chromatography (LC)‐mass spectrometry (MS)/MS directly in intermediates in central carbon metabolism (CCM) to increase temporal resolution. Surprisingly, the...
In a quantitative comparative study, we elucidated the glucose metabolism in fourteen hemiascomycetous yeasts from the Génolevures project. The metabolic networks of these different species were first established by 13 C-labeling data and the inventory of the genomes. This information was subsequently used for metabolic-flux ratio analysis to quantify the intracellular carbon flux distributions...
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