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UK114 is a tumor antigen expressed by various malignant neoplasms. The complete amino acid sequence of UK114 purified from goat liver has been determined by automated Edman degradation of CNBr and endoproteinase Lys-C peptides. The protein contains 137 amino acid residues, which corresponds to a molecular mass of 14 229 Da. MALDI-TOF analysis resulted in a molecular weight of 14 290, suggesting...
A new serine proteinase inhibitor, rapeseed trypsin inhibitor (RTI), has been isolated from rapeseed (Brassica napus var. oleifera) seed. The protein inhibits the catalytic activity of bovine β-trypsin and bovine α-chymotrypsin with apparent dissociation constants of 3.0 x 10 -10 M and 4.1 x 10 -7 M, at pH 8.0 and 21 o C, respectively. The stoichiometry of both...
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