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Fluorescence resonance energy transfer (FRET), which occurs between two luminescent chromophores, can greatly improve the selectivity and sensitivity of a fluorescent assay when a ratiometric signaling with the fluorescence enhancement of the acceptor at the expense of the donor is adopted. In this study, a fluorescence ratiometric detection (FRD) of riboflavin (RF) has been made based on FRET, as...
A simple and sensitive method for amplified chemiluminescent immunoassay has been developed by using multiple signal antibodies functionalized titania nanoparticle as tracer. This nanocomposite was fabricated by sequentially conjugating bovine serum albumin and horseradish peroxidase-labeled antibody onto the surface of titania nanoparticles using glutaraldehyde as the linkage. After a sandwich immuno-binding...
A new label-free amperometric immunosensor was developed for detection of carcinoembryonic antigen (CEA) based on chitosan-ferrocene (CS-Fc) and nano-TiO 2 (CS-Fc+TiO 2 ) complex film and gold nanoparticles–graphene (Au–Gra) nanohybrid. CS-Fc+TiO 2 composite membrane was first modified on a bare glass carbon electrode. Then Au–Gra nanohybrid was formed on the CS-Fc+TiO ...
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