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A fluorescence polarization immunoassay (FPIA) was developed based on a polyclonal antibody for the determination and the qualitative screening analysis of sulfamethazine (SMZ). To optimize the FPIA procedures, three fluorescein-labeled SMZ and sulfamerazine (SMR) were synthesized, and the influence of their structures on the characteristics of FPIA was studied. The optimized FPIA for SMZ had a dynamic...
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