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Crossing the cellular membrane and delivering active pharmaceuticals or biologicals into the cytosol of cells is an essential step in the development of nanomedicines. One of the most important intracellular processes regarding the cellular uptake of biologicals is the endolysosomal pathway. Sophisticated nanocarriers are developed to overcome a major hurdle, the endosomal entrapment, and delivering...
Front Cover: In article number 2200167, Johannes C. Brendel, Anja Traeger, and co‐workers discuss the release of the green fluorescent dye calcein from endosomes, used to study one of the most important intracellular barriers for the uptake of biological substances like genetic material. It includes an overview of various applications as well as the challenges of readily available and commonly used...
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