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A series of continuous cultures was performed to understand the product formation kinetics of recombinant human interferon gamma (rhIFN-γ) in Escherichia coli at different dilution rates ranging from 0.1 to 0.3 h−1 in different media. A T7 promoter-based vector was used for expression of IFN-γ in E. coli BL21 (DE3) cells. The recombinant protein was produced as inclusion bodies, thus allowing a rapid...
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