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We detail a method which allows biochemical quantities of histone proteins to be introduced into a living eukaryotic cell. This method involves absorption of purified proteins into macroplasmodia ofPhysarum polycephalum.Further, sincePhysarummacroplasmodia exist as syncitial culture with completely synchronous nuclei with respect to cell cycle events, proteins may be introduced at specific points...
Increasingly, biochemical analyses of processes that occur within eukaryotic nuclei such as transcription and replication require the construction of specific chromatin substrates. Nucleosome complexes reconstitutedin vitrohave been key elements in a variety of recent studies of polymerase progression andtrans-acting factor binding activities. Reconstituted complexes can be easily constructed from...
Understanding the way genes work requires detailed knowledge of the organization of DNA in the chromatin complex. The difficulties associated with the study of this large macromolecular assembly present an interesting challenge to both biologists and chemists.
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