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The aim of this study is to investigate how the intracellular ROS responds to different degrees of sonoporation. Using a customized platform for 1.5-MHz ultrasound exposure (13.33-μs duration and 0.70-MPa peak negative pressure) and observing the dynamics of sonoporation and intracellular ROS at the single-cell level, we quantified the exogenous molecular uptake and intracellular ROS indicator to...
Plant cells (BY-2 tobacco) are used in this work as a new cellular model for investigating the biological mechanisms behind sonoporation-induced cytotoxic effects. We show that sonoporation may lead to chronic depolarization of plasma membrane and depolarization of mitochondrial membrane: both of which are known to be linked to a cell's programmed cell death protocol. These findings are obtained in-vitro...
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