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Using rat heart sarcolemma and liver plasma membrane vesicles, it has been verified that the transport of leukotriene C 4 (LTC 4 ) across membranes is an ATP-dependent process; the apparent K m for LTC 4 was 150 nM (heart sarcolemma) or 250 nM (liver plasma membrane). S-(2,4-dinitrophenyl)-glutathione (DNP-SG) inhibited LTC 4 uptake into the vesicles dose-dependently...
Incubation of inverted plasma membrane vesicles from rat liver with micromolar concentrations of S-dinitrophenylglutathione (DNP-SG) in the presence of ATP resulted in the uptake of DNP-SG into the vesicles. ATP-dependent DNP-SG accumulation was half-maximal with 9 μM DNP-SG, while the K m for ATP was 320 μM. Glutathione disulfide (GSSG), but not reduced glutathione, inhibited the ATP-dependent...
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