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In this study, we established a Cre/loxP mutant recombination system (Cre/lox71‐66 system) for markerless gene deletion to facilitate our follow‐up rational genetic engineering to the strain Bacillus pumilus W3. This modified method uses two mutant loxP sites, which after recombination creates a double‐mutant loxP site that is poorly recognized by Cre recombinase, facilitating multiple gene deletions...
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