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In order to produce biodiesel from microalgae cultured with abundant seawater, Chlorella sp. was mutated with 137Se-γ ray irradiation and domesticated with f/2 seawater culture medium (salinity=3wt.%) under 15vol.% CO2 stress. Biomass yield of the mutant increased by 25% compared with wild species and lipid content increased to 54.9%. When nitrogen and phosphorus concentrations in the initial substrate...
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