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The transmission of lymphocystis disease virus (LCDV) to gilthead seabream, Sparus aurata L., larvae was investigated using fertilized eggs from a farm with previous reports of lymphocystis disease. LCDV genome was detected by PCR‐hybridization in blood samples from 17.5% of the asymptomatic gilthead seabream broodstock analysed. Using the same methodology, eggs spawned from these animals were LCDV...
Lymphocystis disease virus (LCDV) was detected by PCR-hybridisation in Artemia cysts and metanauplii, and the infectivity of these viral particles was confirmed by inoculation on SAF-1 cells. Viral genome and antigens have been detected by whole-mount in situ hybridisation (ISH) and immunofluorescence assay (IFA) in the digestive tract of nauplii hatched from LCDV-contaminated cysts, but not at the...
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