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Different sterilization doses of the electron beam (E‐beam) will change the properties of biomaterials and affect their clinical application. Acellular porcine cornea (APC) is a promising corneal substitute to alleviate the shortage of corneal resources. The residual DNA was significantly reduced to 18.50 ± 3.19 ng/mg, and the clearance rate of α‐Gal was close to 100% after the treatment with freezing–thawing...