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All methanogens, irrespective of their preferred carbon sources, form coenzyme M (Co-M: 2-mercaptoethane sulphonate) as an intermediate to produce methane (CH 4 ). But the quantification of Co-M in soil is not done before. In this experiment, the method for quantifying Co-M was standardized by HPLC using UV detector and the values of Co-M were compared with CH 4 emission flux and methanogen...
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