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A physical map of Deinococcus radiodurans strain KD8301 was constructed by ordering DNA fragments from genomic DNA digested with the enzymes NotI and PmeI. NotI produced 13 fragments and PmeI four fragments. Junction fragments and multiple enzyme digestion of the genomic DNA, in conjunction with Southern hybridization analysis indicated that KD8301 contains chromosome I (2.6 megabases), chromosome...
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