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The pungent flavor unique in wasabi is due to isothiocyanates including allyl isothiocyanate (AIT) which has outstanding antimicrobial activity. AIT is converted from sinigrin by enzymatic reaction in the presence of water. The optimum moisture content of wasabi root for supercritical CO2 extraction (SCE) at 20 MPa and 35°C was found to be 125% dry basis. In the ranges of pressure (15–25 MPa) and...
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