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In this study, we characterized the immunoglobulin light (IgL) chain gene and examined its expression in the fugu (Takifugu rubripes). The cDNA fragment that partially encodes the IgL chain was isolated by RACE and used as a probe for screening for IgL in a fugu splenic cDNA library. The IgL cDNA sequence that we found consisted of a variable (VL) and a constant (CL) segment. Its structural features...
We have isolated and characterized the cDNA that encodes IgD of fugu (Takifugu rubripes). Though the splicing of μ1 with the δ1 domain was similar to those reported for teleost IgDs, highly unusual and unprecedented domain duplications were found in the constant region of the fugu IgD. The structure of the fugu IgD is like VDJ-μ1-(δ1-δ2-δ3-δ4-δ5-δ6)2-δ7-δm1-δm2. Genomic sequence analysis of the fugu...
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