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The membrane topology of α2/δ subunit was investigated utilizing electrophysiological functional assay and specific anti-α2 antibodies. (a) cRNA encoding a deleted α2/δ subunit was coinjected with α1C subunit of the L-type calcium channel into Xenopus oocytes. The truncated form, lacking the third putative TM domain (α2/δΔTMIII), failed to amplify the expressed inward currents, normally induced by...
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