The Infona portal uses cookies, i.e. strings of text saved by a browser on the user's device. The portal can access those files and use them to remember the user's data, such as their chosen settings (screen view, interface language, etc.), or their login data. By using the Infona portal the user accepts automatic saving and using this information for portal operation purposes. More information on the subject can be found in the Privacy Policy and Terms of Service. By closing this window the user confirms that they have read the information on cookie usage, and they accept the privacy policy and the way cookies are used by the portal. You can change the cookie settings in your browser.
This study aimed to study the immune activity of glutamate dehydrogenase (GDH) of streptococcus suis serotype 2 and detect its activity. METHODS: The GDH gene was cloned and sequenced. Recombinant GDH protein was expressed in a prokaryotic expression system and purified by affinity chromatography. Immunocompetenc of the resulting protein was analyzed by western blotting with anti-GDH monoclonal antibody...
The primers and probes were designed and synthesized according to the conserved VP1 sequences of porcine Sapovirus (SaV), and a TaqMan fluorescence quantitative RT-PCR assay were developed by optimizing the reaction conditions. Results showed that the fluorescence quantitative RT-PCR assay could detect 16.1 copies·µL−1 of plasmid DNA, while the sensitivity of the routine RT-PCR was 1.61 × 103 copies·µL...
To investigate the changes of immuneparameter induced by the infection of Streptococcus suis serotype 2 (SS2) in mini-swine model. The pigs were inoculated with 1 times 107 CFU ml-1/kg of two SS2 strains (ZY05719 and HA9801). Typical clinical symptoms and pathological features were confirmed after infection. Bacterial content in certain organs and changes of white blood cell counts were measured;...
Set the date range to filter the displayed results. You can set a starting date, ending date or both. You can enter the dates manually or choose them from the calendar.