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This study describes the development and evaluation of a specific Legionella pneumophila Taqman duplex real-time PCR (qPCR) for fast and reliable quantification of this human pathogen in suspected man-made water systems. The qPCR assay was 100% specific for all L. pneumophila serogroups 1–15 with a sensitivity of 60 genome units/l and an amplification efficiency of 98%. Amplification inhibitors were...
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