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Affinity detection is a key step of the SELEX screening process for aptamer. Traditional detection depends on the isotope or fluorescence marker in the ssDNA library, but isotope have the danger of radiation and fluorescence is not stable enough in some situations. Therefore, searching for a new, safe and proper detection for the affinity of ssDNA library is needed for the SELEX technology. In the...
The outbreak mechanism of red tide and the rapid reproducing mechanism of red-tide species are not very clear so far. Diatom is one of the main red tide microalgae species. It can utilize the environmental silicon to biosynthesize its nano-silicon cell wall, so its growth and reproduction are closely related to the absorption and bioaccumulation of environmental silicon. Therefore, study on the response...
Diatom Ditylum brightwelli (West) Grunow was cultivated for 5 days, under the environment of low silicon concentration (6 mg/L), high silicon concentration (150 mg/L) and normal silicon concentration (30 mg/L, control ) respectively. Then the plasma membrane of the diatom was extracted and purified by two-phase method. After the analysis of the Na+/K+-ATPase, Ca2+/Mg2+-ATPase and total ATPase activities...
Chemical medicines and antibiotics can kill pathogenic microorganisms in aquiculture animals, but they will lead to environmental pollution and serious food safety problems. Therefore, study on the selection for the disease resistant group of animals is necessary to solve the problem and promote the development of aquaculture. From the view of the relationship between fishes and pathogenic microorganisms,...
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