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The aim of this study was to test the hypothesis that bulls have significant intra-individual differences in the hydraulic conductivity (L p ) and permeability coefficient for glycerol (P s ) of the sperm cell membrane. The permeability parameters were determined at 22, 10, and 0°C of sperm from 7 Holstein Frisian artificial insemination (AI) bulls, using four ejaculates per bull....
The influence of the presence of trehalose or sucrose, the osmolality of the freezing medium, and the cooling rate on success of cryopreservation of bull sperm were investigated. Bull semen was frozen at four different cooling rates varying from 40 to 300 o C/min in standard Tris-egg yolk medium and in hypertonic or isotonic media containing 0.2Mtrehalose or sucrose. Sperm motility, H33258...
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