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Gelsolin is an actin-binding protein that is regulated by the occupancy of multiple calcium-binding sites. We have studied calcium induced conformational changes in the G1–2 and G1–3 sub-domains, and report the binding affinities for the three type II sites. A new probe for G3 has been produced and a K d of 5μM has been measured for calcium in the context of G1–3. The two halves of gelsolin,...
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