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The serine protease gene from a thermophilic fungus Thermoascus aurantiacus var. levisporus, was cloned, sequenced, and expressed in Pichia pastoris and the recombinant protein was characterized. The full-length cDNA of 2,592 bp contains an ORF of 1,482 bp encoding 494 amino acids. Sequence analysis of the deduced amino acid sequence revealed high homology with subtilisin serine proteases. The putative...
In this study, differentially expression genes were isolated from a indica rice cultivar Zhong156 with a race-specific blast resistance gene subjected to the avirulent race ZC15 and the virulent race ZB1 of Magnaporthe grisea at the seedling growth stage. Twenty-five cDNA clones were isolated by using suppression subtractive hybridization combined with reverse Northern blot and confirmed by RT-PCR...
A superoxide dismutase (SOD) gene of Thermoascus aurantiacus var. levisporus, a thermophilic fungus, was cloned, sequenced, and expressed in Pichia pastoris and its gene product was characterized. The coding sequence predicted a 231 residues protein with a unique 35 amino acids extension at the N-terminus indicating a mitochondrial-targeting sequence. The content of Mn was 2.46 μg/mg of protein and...
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