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As we have known, with the plasticizer disturbance in 2011 in Taiwan, long-term exposure to diethyl phthalate (DEP), one of the widely used phthalate esters, can lead to serious health problems. Therefore, a highly sensitive indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) by antigen-coated plate format for DEP in foods was proposed in this paper. The polyclonal antibodies were raised...
A direct competitive chemiluminescent enzyme-linked immunosorbent assay (CL-ELISA) based on polyclonal antibodies with enhanced chemiluminescent detection of dipropyl phthalate (DPrP) has been developed. Hapten with an amino linker introduced on the target molecule was synthesized. Bovine serum albumin was attached to the hapten by a diazotization method to prepare the immunogen. Rabbit antiserum...
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