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Purpose Methanotrophs are an important group of methane (CH4)-oxidizing bacteria in the soil, which act as a major sink for the greenhouse gas, CH4. In grazed grassland, one of the ecologically most sensitive areas is the animal urine patch soil, which is a major source of both nitrate (NO3−) leaching and nitrous oxide (N2O) emissions. Nitrification inhibitors, such as dicyandiamide (DCD), have been...
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