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Previously, we established a method to detect subunit interactions of F 1 -ATPase by the yeast two-hybrid system (Moritani, C.,et al. Biochim. Biophys. Acta1274, 67-72, 1996). Here, we isolated mutants of the γ subunits defective in interaction with the ε subunit by this new procedure to study the molecular basis of coupling mechanisms of the F 1 F 0 -ATPase. Based on the intensities...
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