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This work presents a detailed kinetic study that shows the coupling between the E2→E1 transition and Rb + deocclusion stimulated by Na + in pig-kidney purified Na,K-ATPase. Using rapid mixing techniques, we measured in parallel experiments the decrease in concentration of occluded Rb + and the increase in eosin fluorescence (the formation of E1) as a function of time. The E2→E1...
Despite its similarity with the Na + /K + -ATPase, it has not been possible so far to isolate a K + -occluded state in the H + /K + -ATPase at room temperature. We report here results on the time course of formation of a state containing occluded Rb + (as surrogate for K + ) in H + /K + -ATPase from gastric vesicles at 25°C. Alamethicin...
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