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Background
Durable transgene expression from plasmid DNAs is the key to gene therapy with non‐viral vectors. A comparison of the durability of transgene expression from plasmid DNAs with the CpG‐free and ‐containing backbones is important.
Methods
We constructed plasmid DNAs with the CpG‐containing backbone, various transcription regulatory sequences with and without CpG, and the gene encoding...
BackgroundTransgene expression from plasmid DNA is dependent on the expression efficiency per plasmid and the amount of intranuclear plasmid. In the present study, intranuclear dispositions of two types of plasmid DNAs (i.e. the pCpGfree and pLIVE plasmids) that maintain transgene expression in mouse liver were analyzed. In addition, the relationship between transgene expression and plasmid stability...
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