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Spatial organization of splicing machinery elements in metabolically and functionally different pollen cells during Hyacinthus orientalis pollen grain development was examined by localization of trimethylguanosine (TMG) snRNA and Sm proteins, representing splicing small nuclear ribonucleoproteins, as well as SR splicing factors was investigated. In young pollen grains the level of all labeled antigens...
The content and distribution of the poly(A) RNA and splicing machinery element––TMG snRNA in cells of the Hyacinthus orientalis L. mature embryo sac, during the progamic phase and after fertilization, were investigated. Using fluorescence in situ hybridization and immunofluorescence methods we showed that in the mature unfertilized embryo sac strong signal indicating poly(A) RNA and TMG snRNA appeared...
Summary The article brings forward basic issues related to splicing in plant and animal cells. The results taken up from experiments conducted on generative cells line of animals (oocytes) and plants (meiocytes, microspores and pollen grains) reveal many universal aspects of splicing system organization. Excepting close similarities of biochemical nature, a common feature of both plant and animal...
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