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A real-time TaqMan PCR based on the cytomegalovirus (CMV) polymerase (pol) gene was developed for quantitation of CMV DNA in crude peripheral blood leukocyte (PBL) lysate from stem cell transplantation (SCT) patients. The dynamic range of the assay was between 10 and 4x10 6 copies. Both intra- and inter-assay variability were well within +/-0.25log 10 S.D. Thus, a pooled PBL sample...
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