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We describe a vector and a streamlined procedure for isolating high-producing stable mammalian cell transfectants. The vector encodes theEscherichia coli lacZgene as a reporter. We show that levels of β-galactosidase activity, assayedin situin clonal isolates, can be used to identify clones producing high levels of the protein of interest (in this case, soluble human CD4 protein).
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