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Klebsiella oxytoca has been shown to be able to grow on 1-8mM of tetracyanonickelate (II) [K 2 Ni(CN) 4 ] (TCN), the sole nitrogen source. The biotransformation of 1mM of TCN to ammonia and methane by resting cells of K. oxytoca was confirmed in this study. The utilization of 1mM of TCN was strikingly enhanced when 0.8% of glucose was added into the phosphate buffer containing the...
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