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Mechanically-deboned cod muscle proteins were sequentially hydrolysed using pepsin and a trypsin+chymotrypsin combination, which was followed by passing the digest through a 1kDa equipped tangential flow filtration system; the permeate (<1kDa peptides) was collected as the cod protein hydrolysate (CPH). Reversed-phase high performance liquid chromatography (RP-HPLC) was used to separate the CPH...
The aim of this study was to identify and test efficacy of antioxidant and antihypertensive peptides present in hemp seed protein hydrolysate (HPH), which was produced through simulated gastrointestinal tract digestion of hemp seed proteins. Consecutive fractionation of HPH by reverse-phase HPLC followed by tandem mass spectrometry analysis of active peaks led to identification of 23 short-chain (⩽5...
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