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Investigation was carried out to elucidate the influence of process parameters on the mean particle size of chitosan microspheres produced by water-in-oil (w/o) emulsion crosslinking method. The aqueous chitosan solution was dispersed in the oil phase composed of 40% (v/v) sunflower oil and 60% (v/v) n-hexadecane in the presence of Tween 80 surfactant. The droplets were solidified and hardened by...
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