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We have examined the properties of intramolecular G-quadruplexes in which the G3 tracts are separated by single base loops. The most stable complex contained 1′,2′-dideoxyribose in all three loops, while loops containing T and C were slightly less stable (by about 2°C). Quadruplexes containing loops with single A residues were less stable by 8°C for each T to A substitution. These folded sequences...
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