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Objectives Endogenous adenosine is known to protect the heart. We aimed to develop the method of increasing endogenous adenosine production in non-ischemic heart cells.Design and Methods Isolated rat cardiomyocytes and cultured endothelial cells were incubated with: (1) EHNA--adenosine deaminase inhibitor, (2) EHNA and 5 -iodotubercidine (ITu, adenosine kinase inhibitor), (3) EHNA, ITu and adenine...
Objectives To evaluate the differences in the mechanism of nucleotide breakdown during normothermic and hypothermic, cardioplegic ischemia.Design and Methods Langendorff perfused rat hearts were subjected to: (1) 15 min global ischemia at 37°C followed by 30 min reperfusion and (2) cardioplegic arrest (St. Thomas' No. 1) followed by 6 hr of ischemia at 4°C and 30 min reperfusion. Coronary effluent...
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