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A haploid generation of opposite mating types a and was isolated from sporulating S. cerevisiae industrial yeasts. Haploid yeasts fermenting melibiose and osmotolerant haploids were selected from the C-2 strain used in the production of dried yeasts. It was found that all melibiose-fermenting haploids belong to mating type a. Hybrids were obtained combining features of the parent yeasts G67 and C-2...
The effect of electric force pulses on the survival rate of protoplasts of auxotrophic haploid Saccharomyces cerevisiae yeasts was investigated. Increases of electric force were found to decrease the survival rate of protoplasts which was down to 50-60% at 22.5 kV/cm. 5 kV/cm pulses of electric force increase the frequency of haploid protoplasts fusion by about 50%.
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